|PROJECTE||Tracking and assessing the Risk from Antibiotic resistant genes using Chip technology in surface water Ecosystems (TRACE)|
MINECO - JPI-Water
Leibniz Institute of Photonic Technology, Jena, Germany
Carles Borrego Moré
Dr. José Luis Balcázar, Dr. Marta Villagrasa and Àlex Sànchez
Project TRACE (Tracking and assessing the Risk from Antibiotic resistant genes using Chip technology in surface water Ecosystems, grant Agreement Nº 281347) aims to develop a multiplex DNA Chip-based analysis for the detection of antibiotic resistance genes (ARGs) in both environmental bacteria and aquatic pathogens. To achieve this goal we will study the extent of antibiotic pollution by analyzing up to 56 different antibiotic types as well as the diversity and abundance of ARGs in three aquatic model ecosystems which water is used for both irrigation and recreational purposes: the River Ter (Girona, Spain), the river Tiber/Ostia beach (Roma, Italy) and the Luetsche Lake (Thüringer Wald, Germany). These data will provide both a complete characterization of the microbial resistome in relation to the magnitude of antibiotic pollution in these systems and an inventory of prevalent ARGs to be used as candidate genes for DNA chip design. This DNA-based technology will be then used to track the origin and fate of ARGs in waters used for recreational and irrigation purposes, providing data for studying the potential spreading of resistance genes among environmental bacteria and assessing the risk for human health in relation to exposure to antibiotic resistant organisms. The ICRA team in TRACE is composed of Dr. Carles Borrego (PI), Dr. José Luis Balcázar, Dr. Marta Villagrasa and Alex Sanchez.