The increasing global momentum to address antimicrobial resistance is truly inspiring. Across the globe, hundreds of dedicated individuals are tirelessly working to protect people, animals, and the environment from the threat of drug-resistant diseases. EUJAMRAI2, the second edition of the European Joint Action on Antimicrobial Resistance and Healthcare-Associated Infections, emerges with the ambitious goal of changing the world’s approach to antimicrobial resistance through a One Health perspective. Building on the success of its inaugural edition (2017-2021), the European Union has made an unprecedented investment in the second edition, EU-JAMRAI 2, firmly believing that collaborative efforts among the union’s 30 member countries will elevate Europe as a beacon of best practices. Over the course of 48 months, commencing on January 1st, 2024, more than 120 partners spanning 30 countries, supported by approximately 40 stakeholders, are actively engaged in this joint action. European institutions such as ECDC and EFSA, alongside global bodies including WHO, WOAH, OECD, UNEP, and FAO, contribute their expertise to enrich discussions and ensure alignment with existing initiatives. Furthermore, professionals in human, animal, and environmental health, as well as patient representatives, play integral roles in this collective effort.