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Catalan Institute for Water Research

Research and Innovation for the sustainable use of water

The Biological and Molecular Techniques Unit (UTBM) specializes in the molecular analysis of a wide variety of aqueous matrices (human consumption sources, fluvial biofilm, sediments, wastewater, active sludge) from high quality extraction of nucleic acids (DNA and RNA).

The application of molecular techniques such as PCR and qPCR allows the detection and quantification of any target gene in user samples. The UTBM has specialized in the quantification of resistance genes of a wide range of antibiotic families (ARGs), in line with the compounds detected in the MSU, promoting the transversality of the laboratories that make up the SCTs.

The UTBM also has a Radioactive Facility with non-encapsulated sources, which allows the study of the radionuclides 3H, 14C, 35S using equipment recommended for most cases where a high level of sensitivity is required.

Services

  • Study of bacterial communities
  • Characterization and quantification of genetic elements related to anthropogenic activity
  • Characterization and quantification of the resistome
  • Analysis of acute toxicity (microtox) and mutagenicity (Ames test) in water samples
  • Analysis of radioisotopes (3H, 14C, 35S)

Antibiotic Resistance Genes

  • Sulfonamides

    sull, sulll

  • Macrolides

    ermB

  • Tetracyclines

    tetM, tetW, tetO

  • Beta-lactamases

    blaTEM, blaNDM, blaOXA-46, blaKPC, blaSHV, blaCTX

  • Quinolones

    qnrA, qnrS

  • Polimixines

    mcr1

  • Vancomicina

    vanA

  • Streptomicina

    aadA

Other relevant genes

  • Bacterial community

    16S rDNA

  • Anthropogenic contamination indicator

    intl1

  • Bacterial stress indicator

    recA, soxS

  • Metal resistance

    czcA, pbrT

  • Fusobacterium nucleatum

    fus

  • E.coli

    uidA

  • Archaea community

    16S rDNA

  • Eukaryota community

    18S

  • Cyanophages

    Nv2247, Nv2310, Nv2314

  • Bacteriophages

    T4, crAssphage

Equipment

  • Large volumen centrifuge, Avanti J-26 XPI

  • Scintillation equipment, Tricarb 2910 TR, Perkin-Elmer

  • Molecular Imager, Gel Doc Xr+, Bio-rad

  • Homogenizer, FastPrep 24, MP Biomedicals

  • Microtox, Model 500, SDI Environmental

  • Quantitative Thermal Cycler PCR, CFX96, Bio-rad

  • Conventional Thermal Cycler PCR, 2720, Applied Biosystems

  • Gradient Thermal Cycler PCR, Veriti, Applied Biosystems

  • Qubit, 4.0, Invitrogen

  • Refrigerated centrifuge, 5804 R, Eppendorf

Human team